Antimicrobial susceptibility of plant extracts against human dental caries pathogen

Document Type:Thesis

Subject Area:Biology

Document 1

The challenges include; Increased failure of antibiotics in getting rid of bacteria, financial problems amongst patients in most of the developing countries and unexpected reactions on patients by the antibacterial agents that are administered to the patients. Dental wellbeing plays a very crucial role in the success of a person. A person who is orally okay has got very high chances of succeeding in life. The wellbeing of an individual usually leads to a person making positive and correct life decisions. The decisions that are usually affected by the state of a person's being include The type of nourishment a person settles on, prosperity level of a person, the discourse of an individual and finally an individual’s satisfaction. The reasons include: The process is an inexpensive one, minimal time required for the accomplishment of the task and finally the provision of a clean and safe environment to conduct the test.

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The clean and secure testing environment is made possible via either immunization that is done to the agar plates or the pouring of a liquid to the petri dish which has to be vaccinated. In the disc diffusion method that was settled on, bacteria are usually put on an agar plate that is already loaded with bacteria. Upon the incubation of the plate, if there emerges an area where regardless of the bacteria sprouting it cannot be visible, that zone will be termed as an inhibition zone. The extractions from the plant samples have indicated that there has been an inhibition regarding the growth of the bacteria that is being studied. The usage of the ethanol-methanol solvent is as a result of the ability of the solution to give a consistent and reliable antimicrobial activity.

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As per the current investigation, the plant extracts under study were all of them prepared via water and a solution of organic solvents which would act as the solvents. The reason for settling on water and the organic solution was because of the need to ensure that all the plant extracts that were being tested in this case dissolve on the solvents as per their polarities. However, records indicate that there has been a minimal use the leave extracts of P. guan java, roots of A. The results that were obtained have got some contradictory bits within them. For instance, the results of T. chebula indicated a wide and easily noticeable difference when contrasted with what is existing in the available literature.

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The results, in this case, show that there was minimal effectiveness of the aqueous extracts of T. chebula than its original organic solvent when contrasted with the S. Though the information from the clinical studies available does not possess entire relevance towards the control of caries, the safe usage of the plant extracts towards the curing of varied human diseases show that their application will be appropriate for the caries control. It is also important to note that both the toxic and allergic potentialities of the plant extracts were not included in this study. This automatically implies that no final verdict can be made in this study as regards to the toxic and allergy levels of the extracts. Future studies should focus on the same in a bid to prevent the occurrence of diseases that are termed as periodontal.

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Dental caries, as per the report from the World Health Organization show that whereas the incidences of dental caries in industrialized countries are reducing, there has been a surge of dental caries in the developing nations. mutant 1st 2nd 3rd Mean 1st 2nd 3rd Mean 800gm/ml 7. 23 600gm/ml 5. 17 400gm/ml 3. 27 200gm/ml 2. 1 100gm/ml 0 0 0 0 0 0 0 0 Figure 1: Rosemary Figure 2: Mentha The above table and charts show the effect of rosemary and Mentha essential oils on the bacteria S mutant. aures. The inhibition zone increases with an increase in the amount of the essential oil used. The mean values of the inhibition zones indicate that the bacteria will eventually die with the addition of rosemary essential oil. 100mg/ml or less of both essential oils do not affect the inhibition zone of the bacteria.

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Table 2: (Rothia) Rosemary Mentha Rothia mucilaginosa 1st 2nd 3rd Mean 1st 2nd 3rd Mean 800gm/ml 8. 100mg/ml or less of both essential oils do not affect the inhibition zone of the bacteria. Rosemary Mentha E. coli 1st 2nd 3rd Mean 1st 2nd 3rd Mean 800gm/ml n/a n/a n/a n/a 4 4. 1 600gm/ml n/a 4 n/a 4 3. 63 400gm/ml n/a n/a n/a n/a 2. 3 400gm/ml 3 3. 2 200gm/ml 2 2. 03 100gm/ml 0 0 0 0 0 0 0 0 Table 5: S. sanguis Figure 10: Rosemary Figure 11: Mentha The charts indicate that the inhibition zone of S. sanguis increases with the addition in the concentration of rosemary and Mentha essential oils. For instance, the mean values indicate that rosemary essential oil has the most effect on the S.

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