Drosophila unknown mutant genetic maps

Document Type:Lab Report

Subject Area:Management

Document 1

This implies that the first generation of the viable offspring’s do not show the signs of mutation despite the fact that some of them are carriers of the gene. In the second generation when breeding occurs among the pure breeds three occurs no mutation, however when a carrier and a pure gene breed we get another pure gene and a carrier. morerover when a carrier and a carrier genes breed we achieve a pure gene a carrier and a mutant. Generally mutation among alleles do not show in the first generation and only in rare circumstances are they witnessed. But with subsequent crossing of the individuals would lead to mutation as demonstrated in the project. Of main concern remain the mutant variations on the same drosophila owing to the fact that, there are different: eye colorations, body pixel characteristics, wing shape and sizes.

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As for the case of Ebony drosophila which exhibits different body colorations mutations if the ebony, yellow and brown color which are considered autosomal or recessive as far as ebony and brown colored are concerned and produces a variation of about 3:1 highlighting the wild type allele as the most dominat. due to this conclusion its prudent to assume that drosophila has 4 chromosomes with possible three mutation of the ebony type evident in the table below. DNA SEQUENCING Allele 1 allele 2 allele 3 allele 4 0. 01 jade 5. 01+ beige 1. 6 whitish 53. 6 pinkish 48. 2 red 1. 01+ pink 14. 1 limited 73. 4 jade 67. 2 lilac 30. 3 verlional 78. 9 tan 65. 5 snowy 105. 6 pink 65. 3 maroon 113. 3 ebony. Materials Wild type drosophila, mutant type ebony, plastic vials 1. Involve the use of a fly nap to anesthetize them. utelize a sterile swab to wipe the inner part of the inner rim of the fly nap for life assurance of the fly being used.

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Take the swob and press the non-cotton end into the plug. Close the phial. Guarantee the swob is inside. Use sex as a determinant. Examine the males and females for wild and ebony phenotypic characteristics. Record the statistics you have acquired. Results: Cross 1 ebony males X wild females male female total Wild type 68 66 134 ebony 28 23 51 total 96 89 Cross 2 Wild type male X ebony females male female total Wild type 14 13 27 ebony 3 7 10 total 17 20 Cross 3 wild type male x ebony females. male female total Wild type 40 37 27 ebony 11 10 21 total 51 47 Cross 4 wild type male X ebony females Male female total Wild type 36 35 71 ebony 9 9 18 total 45 44 Cross 5 wild type male X ebony females Male female Total Wild type 181 181 362 ebony 45 35 80 total 226 216 Cross 6 wild type male X ebony females Male female total Wild type 188 176 364 ebony 46 56 102 total 234 232   Discussion: We collected our data for a period of 2 months accumulating about 5 data sets with the sixth one coming a little bit later than expected.

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The way that arrangement of information is disregarded and the other acknowledged could be clarified by cross 5 which was dismissed because of high chi square esteem. We realized that due to lack of relationship between ebony body colorations and sex transmission, there was accuracy of data since there was minimal difference existing between the crosses provided. Mutations involving ebony as far as the group members agreed was developed from an autosomal chromosome gene qualifying Mendelian succession theory with a variation of about: 3:1with wild type drosophila being dominant. It was however noticed that some crosses did not produce viable results true to exposure to very minimal temperatures or high temperatures that did not encourage the development of the flies hence false results indications.

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