Vitek 2 report

Document Type:Lab Report

Subject Area:Management

Document 1

Although, blood culture requires an estimate of four- twenty four hours of incubation time, but additional time of twenty four-forty eight hours is also needed for biochemical tests in order to identify bacteria and determine the susceptibility of such bacteria to anti-microbial agents. The appropriate time required to obtain a culture result is much longer where there is low bacterial infections with a slow growth rate of the bacteria. If there is a faster reporting of bacterial identification and susceptibility, the results that will be obtained will have both clinical and financial benefits. The automated blood culture and the automated identification and susceptibility testing systems have been available for the quite a number of years. The possibility of combining these two automated systems to achieve rapid identification and susceptibility testing by inoculation of the two systems when samples are used taken directly from positive blood culture bottles is very high and it will provide the best results.

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The product’s name was changed in 1977 and soon became known as VITEK meaning ‘‘life technology. ’’ There were introduction of additional tests which required pure microbial culture for inoculation. The technologies were surfaced in the 1980s when the systems were under continued development in the area of industrial microbiology which led to a creation of a separate division. McDonald Douglas decided to quit the market in the late 1980 and in the 1989; VITEK became part of the bioMerieux Group which was best known as the developer of rapid microbiology diagnostic test. The system itself is integrated and a modular system that consists of very basic components. They provide an option of automatic process that is carried out by the use laboratory instruments like the pipette and dissolving the reagents for antimicrobial susceptibility testing.

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How the test is performed in the Lab VITEK 2 system is highly sophisticated so the test is set up in a simple manner. The main steps are sub -culturing for the isolation of pure culture, Gram stains, oxidase, and coagulase or carrying out a catalase test. The organism suspension is prepared in a saline environment. A Gram stain and colonial morphology are performed so that the appropriate test ID card is used. The VITEK programmed computer determines when a well is positive based on the broadcast of light measured by an optical scanner. When patterns occur, they are analysed automatically. After incubation cycle, identification is printed by the data terminal for each ID card in the reader. The ID cards can employ many biochemical tests but the unique environment combined with short incubation may produce results that may differ from those appearing in published material of other methods.

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The analysis of the results Identification of the Gram- negative bacilli On the identification of the Gram-negative bacilli, Acinetobacter and non-fermenters were identified by the direct method. Antimicrobial susceptibility testing of Gram-positive cocci The isolates were excluded from antibiotic susceptibility testing due to inconsistent direct identification and susceptibility patterns. Therefore, only 13 Staphylococcus spp. were assayed for susceptibility to 9 antimicrobial agents, with a total of 117 tests. The concordant rates between the two methods ranged from 76. 9 to 100. "Multidrug-resistant Candida auris misidentified as Candida haemulonii: characterization by matrix-assisted laser desorption ionization–time of flight mass spectrometry and DNA sequencing and its antifungal susceptibility profile variability by Vitek 2, CLSI broth microdilution, and Etest method. " Journal of clinical microbiology 53. Machen, Alexandra, Tim Drake, and Yun F.

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